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Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1 ArchiMer
Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
Background: Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. Results: The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant...
Tipo: Text Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf
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Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens ArchiMer
Renault, Tristan; Tchaleu, Gwenaelle; Faury, Nicole; Moreau, Pierrick; Segarra, Amelie; Barbosa-solomieu, Valerie; Lapegue, Sylvie.
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest....
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00172/28306/26582.pdf
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Mass mortality in bivalves and the intricate case of the Pacific oyster, Crassostrea gigas ArchiMer
Barbosa-solomieu, Valerie; Renault, Tristan; Travers, Marie-agnes.
Massive mortality outbreaks in cultured bivalves have been reported worldwide and they have been associated with infection by a range of viral and bacterial pathogens. Due to their economic and social impact, these episodes constitute a particularly sensitive issue in Pacific oyster (Crassostrea gigas) production. Since 2008, mortality outbreaks affecting C. gigas have increased in terms of intensity and geographic distribution. Epidemiologic surveys have lead to the incrimination of pathogens, specifically OsHV-1 and bacteria of the Vibrio genus, in particular Vibrio aestuarianus. Pathogen diversity may partially account for the variability in the outcome of infections. Host factors (age, reproductive status…) including their genetic background that has...
Tipo: Text Palavras-chave: Pacific oyster; Spat; OsHV-1; Vibrio aestuarianus; Mortality.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00274/38534/37051.pdf
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